Simple Plex Human Osteoprotegerin/OPG Cartridge

Name: Simple Plex Human Osteoprotegerin/OPG Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000521

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000521

Simple Plex Human Osteoprotegerin/TNFRSF11B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	1.17 pg/mL
Assay Range:	4.19 - 40,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human Osteoprotegerin/OPG Cartridge Summary:	Simple Plex assay is for the detection of human Osteoprotegrin (OPG)/TNFRSF11B in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Osteoprotegerin/OPG Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human Osteoprotegerin/OPG Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human OPG.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one OPG assay

Inter-Assay Precision (Precision between assays) Replicates of each OPG control were tested in multiple assays performed by at least three technicians using two lots of reagents for human OPG

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	26	26
Mean (pg/mL)	231	12102	242	11197
Standard Deviation	16.3	976	23.5	1133
CV%	7.1	8.1	9.7	10.1

Linearity

Samples containing and/or spiked with high concentrations of human OPG were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human Osteoprotegerin/OPG Cartridge

	Simple Plex Human OPG Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human OPG Assay. Assay range is 4.20-40,000 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin (OPG), also known as OCIF and TNFRSF11B, is a secreted TNF superfamily receptor that regulates bone density. OPG is widely expressed and constitutively released as a homodimer by mesenchymal stem cells, fibroblasts, and endothelial cells. OPG functions as a decoy receptor for TRANCE/RANK L and TRAIL. OPG regulates the cell surface expression of TRANCE and its ability to stimulate osteoclastogenesis. Dysregulation of OPG/TRANCE interactions can lead to juvenile Paget’s disease, osteoporosis, bone loss, and vascular calcification.

Item	Value
Entrez Gene IDs	4982 (Human); 18383 (Mouse)
Alternate Names	OCIF, OCIFMGC29565, OPG, OPGtumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin, TNFRSF11B, TR1, tumor necrosis factor receptor superfamily, member 11b

Compatible Instruments

Ella - Automated Immunoassay Platform

Ella Instrument. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Osteoprotegerin/OPG Cartridge

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Protocols & Manuals

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