Simple Plex Human MMP-9 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000661
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human MMP-9 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) ach control was tested multiple times in one MMP-9 assay.
Inter-Assay Precision (Precision between assays) Replicates of each MMP-9 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human MMP-9.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 24 |
| Mean (pg/mL) | 979 | 50,388 | 974 | 48,013 |
| Standard Deviation | 48.2 | 3412 | 84.4 | 4337 |
| CV% | 4.9 | 6.8 | 8.7 | 9.0 |
Recovery for Simple Plex Human MMP-9 Cartridge
Recovery for the human MMP-9 assays was evaluated at three different spiked concentrations of MMP-9 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=2) | 100 | 96-104 |
Linearity
Samples containing and/or spiked with high concentrations of human MMP-9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human MMP-9 Cartridge
Simple Plex Human MMP-9 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human MMP-9 Assay. Assay range is 40.0-152,700 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: MMP-9
MMP-9 (Gelatinase B) is a metalloproteinase that degrades multiple extracellular matrix components including Gelatin, Collagens III, IV, V, and XI, Elastin, Nidogen-1, and Vitronectin. It also cleaves a variety of chemokines and growth factors (e.g. IL-1 beta, CXCL8/IL-8, CXCL7, CXCL4, CXCL1, Latent TGF-beta, membrane bound TNF-alpha, VEGF, and FGF basic), Amyloid beta peptide, Substance P, and Myelin Basic Protein. MMP-9 can trigger signaling through various transmembrane proteins or inhibit signaling by inducing their shedding from the cell surface (e.g. CD44, E-Cadherin, Integrins, ICAM-1, and CD25/IL-2 R alpha). The activity of MMP-9 is regulated by its association with the TIMP-1 protease inhibitor. Circulating levels of MMP-9 are elevated in many inflammatory disorders including intraluminal thrombus formation, atherosclerosis, Crohn’s disease, hepatitis C virus infection, colorectal cancer, and Duchenne muscular dystrophy.
Long Name
Alternate Names
Gene Symbol
Additional MMP-9 Products
Product Documents for Simple Plex Human MMP-9 Cartridge
Product Specific Notices for Simple Plex Human MMP-9 Cartridge
For research use only