Are mouse or rat CXCL8/IL-8 products offered?

Name: Simple Plex Human IL-8 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000230

Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

Simple Plex Human IL-8 Cartridge

Catalog # SPCKB-PS-000230 | ProteinSimple a Bio-Techne Brand

Citations (1)

Product Datasheet / COA

Simple Plex Human IL-8 assay kit for use on Ella automated benchtop ELISA. Contains cartridge, sample diluent SD13, and wash buffer.

Need a different panel or sample type? Customize your cartridge here.

Key Product Details

Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity:	0.08 pg/mL
Assay Range:	0.19 - 1,804 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)
Simple Plex Human IL-8 Cartridge Summary:	Simple Plex assay for the detection of human Interleukin 8 (IL-8) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human IL-8 Cartridge

Technical Data
Citations (1) / Reviews / FAQs

Product Specifications for Simple Plex Human IL-8 Cartridge

Reactivity	Human
Specificity	Natural and recombinant human IL-8.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	10	10
Mean (pg/mL)	28.7	1362	27.3	1463
Standard Deviation	1.41	89.8	1.41	125
CV%	4.9	6.6	5.2	8.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=5)	89	75-102
Heparin Plasma (n=5)	95	88-102
Serum (n=3)	92	88-97

Linearity

Samples containing and/or spiked with high concentrations of human IL-8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human IL-8 Cartridge

	Human IL-8 Ella Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD163

CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.

Item	Value
Entrez Gene IDs	9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names	CD163, CD163 molecule, CD_antigen: CD163, GHI/61, HbSR, Hemoglobin scavenger receptor, M130, MM130, RM3/1, SCARI1, Soluble CD163, macrophage-associated antigen, sCD163, scavenger receptor cysteine-rich type 1 protein M130

Citations for Simple Plex Human IL-8 Cartridge (1)

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Citations are publications that use Bio-Techne products. Selected citations for Simple Plex Human IL-8 Cartridge include:

S Morselli, C Foschi, L Laghi, S Zagonari, G Patuelli, T Camboni, C Ceccarani, C Consolandi, ME Djusse, MF Pedna, A Marangoni, M Severgnini, V Sambri Torquetenovirus in pregnancy: Correlation with vaginal microbiome, metabolome and pro-inflammatory cytokines Frontiers in Microbiology, 2022;13(0):998849.. 998849
PMID: 36160242

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FAQs for Simple Plex Human IL-8 Cartridge

Are mouse or rat CXCL8/IL-8 products offered?

Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.