Simple Plex Human IL-8 Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000230
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-8 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-8 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-8 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-8.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 10 | 10 |
| Mean (pg/mL) | 28.7 | 1362 | 27.3 | 1463 |
| Standard Deviation | 1.41 | 89.8 | 1.41 | 125.0 |
| CV% | 4.9 | 6.6 | 5.2 | 8.6 |
Recovery for Simple Plex Human IL-8 Cartridge
Recovery for the human IL-8 assays was evaluated at three different spiked concentrations of IL-8 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=5) | 89 | 75-102 |
| Heparin Plasma (n=5) | 95 | 88-102 |
| Serum (n=3) | 92 | 88-97 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IL-8 Cartridge
Simple Plex Human IL-8 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-8 Assay. Assay range is 0.19-1804 pg/mLPreparation and Storage
Shipping
Stability & Storage
Background: IL-8/CXCL8
Interleukin-8 (IL-8), also known as CXCL8, is a chemokine that is upregulated at sites of inflammation where it promotes neutrophil infiltration and activation. IL-8 can form homodimers and heterodimers with CXCL4/PF4. Its bioactivity is regulated by proteolytic truncations, citrullination, and the decoy receptor DARC. IL-8 signals through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. IL-8 also binds to Serpin A1/alpha-1 Antitrypsin which prevents IL-8 interaction with CXCR1.
Long Name
Alternate Names
Gene Symbol
Additional IL-8/CXCL8 Products
Product Documents for Simple Plex Human IL-8 Cartridge
Product Specific Notices for Simple Plex Human IL-8 Cartridge
For research use only