Simple Plex Human IL-8 Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000230
Need a different panel or sample type? Customize your cartridge here.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-8 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-8 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-8 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-8.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 10 | 10 |
| Mean (pg/mL) | 28.7 | 1362 | 27.3 | 1463 |
| Standard Deviation | 1.41 | 89.8 | 1.41 | 125.0 |
| CV% | 4.9 | 6.6 | 5.2 | 8.6 |
Recovery for Simple Plex Human IL-8 Cartridge
Recovery for the human IL-8 assays was evaluated at three different spiked concentrations of IL-8 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=5) | 89 | 75-102 |
| Heparin Plasma (n=5) | 95 | 88-102 |
| Serum (n=3) | 92 | 88-97 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IL-8 Cartridge
Simple Plex Human IL-8 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-8 Assay. Assay range is 0.19-1804 pg/mLPreparation and Storage
Shipping
Stability & Storage
Background: CD163
CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.
Alternate Names
Gene Symbol
Additional CD163 Products
Product Documents for Simple Plex Human IL-8 Cartridge
Product Specific Notices for Simple Plex Human IL-8 Cartridge
For research use only