Simple Plex Human IL-4 Cartridge, 2nd Generation
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002800
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-4 Cartridge, 2nd Generation Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-4 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-4 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-4.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 46 | 46 |
| Mean (pg/mL) | 15.9 | 621 | 13.5 | 631 |
| Standard Deviation | 1.20 | 60.8 | 1.55 | 77.1 |
| CV% | 7.5 | 9.8 | 11.5 | 12.2 |
Recovery for Simple Plex Human IL-4 Cartridge, 2nd Generation
Recovery for the human IL-4 assays was evaluated at three different spiked concentrations of IL-4 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 88 | 83-92 |
| Heparin Plasma (n=4) | 87 | 84-89 |
| Serum (n=4) | 96 | 94-99 |
Linearity
<div style="box-sizing: border-box; color: rgb(51, 51, 51); font-family: arial, sans-serif; font-size: 14px;">Samples containing and/or spiked with high concentrations of human IL-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.</div>
Scientific Data Images for Simple Plex Human IL-4 Cartridge, 2nd Generation
Simple Plex Human IL-4 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-4 Assay. Assay range is 0.53-2020 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-4
IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-4 Products
Product Documents for Simple Plex Human IL-4 Cartridge, 2nd Generation
Product Specific Notices for Simple Plex Human IL-4 Cartridge, 2nd Generation
For research use only