Simple Plex Human IL-17/IL17A Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000445
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-17/IL17A Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-17A assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-17A control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-17A.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 11 | 12 |
| Mean (pg/mL) | 57.7 | 2715 | 58.0 | 2513 |
| Standard Deviation | 2.80 | 108 | 3.38 | 188 |
| CV% | 4.9 | 4.0 | 5.8 | 7.5 |
Recovery for Simple Plex Human IL-17/IL17A Cartridge
Recovery for the human IL-17A assays was evaluated at three different spiked concentrations of IL-17A protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 86 | 64-111 |
| Heparin Plasma (n=4) | 92 | 87-98 |
| Serum (n=4) | 93 | 86-104 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-17A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human IL-17/IL17A Cartridge
Simple Plex Human IL-17A Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-17A Assay. Assay range is 1.05-10,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-17/IL-17A
IL-17/IL-17A is a homodimeric cytokine that is secreted by IL-23 activated Th17 and Tc17 cells. It is upregulated during inflammation and contributes to disease severity by enhancing neutrophil infiltration and the production of pro-inflammatory chemokines. IL-17A can additionally form bioactive heterodimers with IL-17F. IL-17A and the IL-17A/F heterodimer signal through widely expressed receptor complexes containing IL-17 RA and IL-17 RC.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-17/IL-17A Products
Product Documents for Simple Plex Human IL-17/IL17A Cartridge
Product Specific Notices for Simple Plex Human IL-17/IL17A Cartridge
For research use only