Simple Plex Human E-Selectin/CD62E Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000457
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human E-Selectin/CD62E Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one E-Selectin assay.
Inter-Assay Precision (Precision between assays) Replicates of each E-Selectin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human E-Selectin.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 17 | 19 |
| Mean (pg/mL) | 156 | 7614 | 140 | 6802 |
| Standard Deviation | 9.73 | 461 | 12.0 | 642 |
| CV% | 6.2 | 6.1 | 8.6 | 9.4 |
Recovery for Simple Plex Human E-Selectin/CD62E Cartridge
Recovery for the human E-Selectin assays was evaluated at three different spiked concentrations of E-Selectin protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=7) | 104 | 96-109 |
Linearity
Samples containing and/or spiked with high concentrations of human E-Selectin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human E-Selectin/CD62E Cartridge
Simple Plex Human E-Selectin Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human E-Selectin Assay. Assay range is 2.56-24,460 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: E-Selectin/CD62E
E-Selectin, also known as CD62E, is expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. It is a lectin that binds to sialylated or fucosylated molecules on neutrophils, monocytes, and a subset of memory T cells. E-Selectin mediates the initial attachment of circulating leukocytes to the blood vessel wall during inflammation. This interaction permits leukocytes to roll along the vascular endothelium. Subsequent ICAM-1 and VCAM-1 mediated adhesion leads to extravasation of the leukocyte through the blood vessel wall into the extracellular matrix tissue.
Alternate Names
Gene Symbol
Additional E-Selectin/CD62E Products
Product Documents for Simple Plex Human E-Selectin/CD62E Cartridge
Product Specific Notices for Simple Plex Human E-Selectin/CD62E Cartridge
For research use only