Simple Plex Human E-Cadherin Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000208
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human E-Cadherin Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one E-Cadherin assay.
Inter-Assay Precision (Precision between assays) Replicates of each E-Cadherin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human E-Cadherin.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 24 | 23 |
| Mean (pg/mL) | 75.0 | 3596 | 67.7 | 3841 |
| Standard Deviation | 1.79 | 134 | 6.83 | 399 |
| CV% | 2.4 | 3.7 | 10.1 | 10.4 |
Recovery for Simple Plex Human E-Cadherin Cartridge
Recovery for the human E-Cadherin assays was evaluated at three different spiked concentrations of E-Cadherin protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 100 | 94-107 |
Linearity
Samples containing and/or spiked with high concentrations of human E-Cadherin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human E-Cadherin Cartridge
Simple Plex Human E-Cadherin Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human E-Cadherin Assay. Assay range is 9.92-15140 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: E-Cadherin
E-Cadherin/Cadherin-1 (ECAD) Is a transmembrane cell adhesion protein. It forms homophilic clusters between epithelial cells and is a critical component of adherens junctions. These junctions are critical for the maintenance of the epithelial barrier and epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition (EMT) and cell migration, and E-Cadherin loss contributes to cancer metastasis. The shed ECD is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation.
Alternate Names
Gene Symbol
Additional E-Cadherin Products
Product Documents for Simple Plex Human E-Cadherin Cartridge
Product Specific Notices for Simple Plex Human E-Cadherin Cartridge
For research use only