Simple Plex Human Angiopoietin-like 3 Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-008169
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Angiopoietin-like 3 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 41 | 42 |
| Mean (pg/mL) | 76.4 | 3522 | 74.8 | 3834 |
| Standard Deviation | 2.50 | 186 | 8.47 | 459 |
| CV% | 3.3 | 5.3 | 11.3 | 12.0 |
Recovery for Simple Plex Human Angiopoietin-like 3 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 105 | 101-109 |
Linearity
Samples containing and/or spiked with high concentrations of human ANGPTL3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Angiopoietin-like 3 Cartridge
Human Angiopoietin-like 3 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Angiopoietin-like Protein 3/ANGPTL3
Angiopoietin-like Protein 3 (ANGPTL3) is a secreted glycoprotein that is primarily expressed in the liver. Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N- and C-terminal fragments containing the coiled coil domain and fibrinogen-like domains, respectively. ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids. It promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake. ANGPTL3 is upregulated by LXR agonists and downregulated by Insulin, Leptin, and agonists of TR beta or PPAR beta. Its fibrinogen-like domain interacts with Integrin alphaV beta3 to induce endothelial cell adhesion, migration, and neovascularization. ANGPTL3 also promotes the expansion of hematopoietic stem cells.
Alternate Names
Gene Symbol
Additional Angiopoietin-like Protein 3/ANGPTL3 Products
- All Products for Angiopoietin-like Protein 3/ANGPTL3
- Angiopoietin-like Protein 3/ANGPTL3 cDNA Clones
- Angiopoietin-like Protein 3/ANGPTL3 ELISA Kits
- Angiopoietin-like Protein 3/ANGPTL3 Lysates
- Angiopoietin-like Protein 3/ANGPTL3 Primary Antibodies
- Angiopoietin-like Protein 3/ANGPTL3 Proteins and Enzymes
- Angiopoietin-like Protein 3/ANGPTL3 Simple Plex
Product Documents for Simple Plex Human Angiopoietin-like 3 Cartridge
Product Specific Notices for Simple Plex Human Angiopoietin-like 3 Cartridge
For research use only