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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

HRP

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Rabbit anti-Human IgM Fc5u Secondary Antibody was produced by repeated immunization with Human IgM Fc5u fragment in rabbit.

Specificity

Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgM and Human Serum

Scientific Data Images for Rabbit anti-Human IgM Heavy Chain Secondary Antibody [HRP]

ELISA: Rabbit anti-Human IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73615] - ELISA Results of Rabbit anti-Human IgM Heavy Chain Secondary antibody [HRP]tested against purified Human IgM F(c)5u HRP. Each well was coated in duplicate with 1.0 ug of Human IgM F(c)5u. The working dilution is 1:43,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.

Applications for Rabbit anti-Human IgM Heavy Chain Secondary Antibody [HRP]

Application
Recommended Usage

ELISA

1:15000 - 1:60000

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1:2000 - 1:10000
Application Notes
This product has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: IgM Heavy Chain

Alternate Names

Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain

Gene Symbol

IGHM

Additional IgM Heavy Chain Products

Product Documents for Rabbit anti-Human IgM Heavy Chain Secondary Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit anti-Human IgM Heavy Chain Secondary Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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