Goat F(ab')2 anti-Mouse IgM Heavy Chain Secondary Antibody [PE]
Novus Biologicals, part of Bio-Techne | Catalog # NB120-5932
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Goat F(ab')2 anti-Mouse IgM Heavy Chain Secondary Antibody was produced by repeated immunization with Mouse IgM heavy chain in goat.
Specificity
No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). No reaction was observed against other mouse heavy or light chain proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgM, and Mouse Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgM, and Mouse Serum
Applications for Goat F(ab')2 anti-Mouse IgM Heavy Chain Secondary Antibody [PE]
Application
Recommended Usage
Flow Cytometry
1:100 - 1:250
Immunocytochemistry/ Immunofluorescence
1:100 - 1:250
Application Notes
This product has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: IgM Heavy Chain
Alternate Names
Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene Symbol
IGHM
Additional IgM Heavy Chain Products
Product Documents for Goat F(ab')2 anti-Mouse IgM Heavy Chain Secondary Antibody [PE]
Product Specific Notices for Goat F(ab')2 anti-Mouse IgM Heavy Chain Secondary Antibody [PE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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