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Goat anti-Mouse IgG/IgA/IgM/IgD (H+L) Secondary Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69510

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NBP2-69510

Key Product Details

Species Reactivity

Mouse

Applications

Immunoelectrophoresis, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Purified polyclonal mouse IgG and pools of homogenous IgG, IgA, IgM and IgD. Freund's complete adjuvant is used in the first step of the immunization procedure.

Reactivity Notes

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Specificity

The reactivity of the antiserum is directed to the heavy chains of immunoglobulin isotypes IgG, IgA, IgM and IgD. The antiserum also contains antibodies to common determinants and to the surface determinants of the common Fab as tested by immunoelectrophoresis and double radial immunodiffusion. It does not react with any non-Ig proteins in mouse serum.

Clonality

Polyclonal

Host

Goat

Description

The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Applications for Goat anti-Mouse IgG/IgA/IgM/IgD (H+L) Secondary Antibody

Application
Recommended Usage

Immunoelectrophoresis

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunoprecipitation. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion use a rosette arrangement with 10 ul antiserum in a 3 mm diameter center well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells..

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Reconstitution

Reconstitute by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2).

Formulation

Whole antiserum

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG/IgA/IgM/IgD (H+L)

Additional IgG/IgA/IgM/IgD (H+L) Products

Product Documents for Goat anti-Mouse IgG/IgA/IgM/IgD (H+L) Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Mouse IgG/IgA/IgM/IgD (H+L) Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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