Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68560

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NBP2-68560
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed

Concentration

LYOPH mg/ml

View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG, whole molecule

Specificity

Rat pre-absorbed to minimize cross-reactivity with rat immunoglobulins and is therefore of particular value in detecting mouse primary antibodies bound to rat tissues.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) Flow Cytometry: Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] - (Pre-adsorbed) [NBP2-68560] -

Flow Cytometry: Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] - (Pre-adsorbed) [NBP2-68560] -

Flow Cytometry: Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] - (Pre-adsorbed) [NBP2-68560] - Figure A. FITC conjugated Rat anti Human CD8 and isotype control detected with Goat anti Mouse IgG: RP. Figure B. FITC conjugated Rat anti Human CD8 nd Mouse anti Human CD45RB detected with Goat anti Mouse IgG RPE). All experiments performed on red cell lysed human blood gated on single lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Applications for Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

Application
Recommended Usage

Flow Cytometry

1:5

Formulation, Preparation, and Storage

Purification

Affinity purified

Reconstitution

Reconstitute with 1.0 ml distilled water. Store at 4C.

Formulation

PBS, 1% Bovine Serum Albumin

Format

Pre-adsorbed

Preservative

0.09% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

Product Datasheets

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Product Specific Notices for Goat anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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