Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62585
![Western Blot: Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [NBP2-62585]](https://resources.bio-techne.com/images/products/Goat-F-ab-2-anti-Mouse-IgG-H+L-Secondary-Antibody-Western-Blot-NBP2-62585-img0001.jpg "Western Blot: Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [NBP2-62585]")
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody was produced by repeated immunization with Mouse IgG whole molecule in goat.
Specificity
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG and Mouse Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody
Western Blot: Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [NBP2-62585]
Western Blot: Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [NBP2-62585] - Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Observed/Predicted size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None. Image from the HRP version of this antibody.
Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody
Dot Blot Results of Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody. Mouse IgG [p/n 010-0102] (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: F(ab')2 Goat Anti-Mouse IgG at 1.0ug/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP at 1ug/mL for 30 mins at RT. Block: BlockOut Buffer at RT for 30 mins. Exposure: 1 sec.
Dot Blot: Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [NBP2-62585] -
Dot Blot Results of F(ab')2 Goat Anti-Mouse IgG Antibody. Mouse IgG [p/n 010-0102] (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: F(ab')2 Goat Anti-Mouse IgG at 1.0µg/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP at 1µg/mL for 30 mins at RT. Block: BlockOut Buffer [p/n MB-073] at RT for 30 mins. Exposure: 1 sec.Applications for Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:2000 - 1:10000
Application Notes
This product has been tested by ELISA, SDS-PAGE, dot blot, and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 ug of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG (H+L)
The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).
References
1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211
2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78
3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268
4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619
Additional IgG (H+L) Products
Product Documents for Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody
Product Specific Notices for Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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