Rabbit anti-Llama IgG (H+L) Secondary Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30113

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NBP1-30113
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Key Product Details

Species Reactivity

Llama

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

Purified Llama IgG

Reactivity Notes

Llama

Specificity

This rabbit anti-llama secondary antibody recognizes both heavy and light chains

Clonality

Polyclonal

Host

Rabbit

Description

Enquire for availability of conjugated forms of this secondary antibody.

Scientific Data Images for Rabbit anti-Llama IgG (H+L) Secondary Antibody

Western Blot: Rabbit anti-Llama IgG (H+L) Secondary Antibody [NBP1-30113]

Western Blot: Rabbit anti-Llama IgG (H+L) Secondary Antibody [NBP1-30113]

Western Blot: Rabbit anti-Llama IgG (H+L) Secondary Antibody [NBP1-30113] - Serial dilution of rabbit anti-Llama antibody blot with Rabbit anti-Llama IgG (H+L) Secondary Antibody. Optimal dilution for use of this antibody is 1:30,000. This antibody labels both heavy and light chain of Llama IgG.

Applications for Rabbit anti-Llama IgG (H+L) Secondary Antibody

Application
Recommended Usage

Western Blot

1:30000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS/HEPES/BSA buffer @ pH: 7.4. Contains 30% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Rabbit anti-Llama IgG (H+L) Secondary Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rabbit anti-Llama IgG (H+L) Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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