Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

Novus Biologicals, part of Bio-Techne | Catalog # NB7602

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB7602
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Dot Blot, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Cy5 (Excitation = 649 nm, Emission = 666 nm)

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG whole molecule

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat, Rabbit and Sheep Serum Proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) [NB7602] - Western Blot of Goat anti-Mouse IgG (H+L) Secondary Antibody [Rhodamine]. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [Rhodamine] at 1:1000 for 60 min at RT.Block: Fluorescent blocking buffer for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG.Other band(s): none.. Using the Rhodamine format of this antibody.
Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed) [NB7602] - 0.62ng
Primary antibody: none
Secondary antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [Rhodamine] at 1:1000 for 60 min at RT.
Block: Fluorescent blocking buffer for 60 min at RT.. Using the Rhodamine format of this antibody.

Applications for Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

Application
Recommended Usage

Flow Cytometry

1:500-1:2500

Fluorophore-linked immunosorbent assay

1:10000-1:50000

Immunocytochemistry/ Immunofluorescence

1:1000-1:5000
Application Notes
This product is designed for ICC/IF, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Reviewed Applications

Read 3 reviews rated 4.3 using NB7602 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Format

Pre-adsorbed

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy5] (Pre-adsorbed)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.