Key Product Details

Species Reactivity

Bovine

Applications

ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # IL-A2

Format

BSA Free

Concentration

1 mg/ml

View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

Purified bovine IgG

Specificity

Mouse anti Bovine IgG, clone IL-A2, is specific for bovine IgG. The specificity of this antibody for bovine IgG1 and IgG2 has been demonstrated by ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Mouse anti-Bovine IgG (H+L) Secondary Antibody (IL-A2) (BSA Free)

Mouse anti-Bovine IgG (H+L) Secondary Antibody (IL-A2) ELISA: Mouse anti-Bovine IgG (H+L) Secondary Antibody (IL-A2) [NBP2-68413] -

ELISA: Mouse anti-Bovine IgG (H+L) Secondary Antibody (IL-A2) [NBP2-68413] -

ELISA: Mouse anti-Bovine IgG (H+L) Secondary Antibody (IL-A2) [NBP2-68413] - Bovine IgG detected using Mouse anti Bovine IgG and Rabbit F(ab')2 anti Mouse IgG:HRP as the detection reagents.

Applications for Mouse anti-Bovine IgG (H+L) Secondary Antibody (IL-A2) (BSA Free)

Application
Recommended Usage

ELISA

1:2500 - 1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Mouse anti-Bovine IgG (H+L) Secondary Antibody (IL-A2) (BSA Free)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Mouse anti-Bovine IgG (H+L) Secondary Antibody (IL-A2) (BSA Free)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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