Goat anti-Human IgG Fc Secondary Antibody [DyLight 488]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-74981G
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Chemiluminescence Immunoassay, ELISA, Flow Cytometry, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunomicroscopy, Western Blot
Label
DyLight 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Purified Human IgG, Fc fragment
Reactivity Notes
Based on IEP, this antibody reacts with heavy (gamma) chains on human IgG.
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on human IgG. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with 0 IgG serum proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for Goat anti-Human IgG Fc Secondary Antibody [DyLight 488]
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
1:10 - 1:500
Immunomicroscopy
1:10 - 1:500
Western Blot
1:100-1:2000
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP1-74981. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IgG Fc
UniProt
Additional IgG Fc Products
Product Documents for Goat anti-Human IgG Fc Secondary Antibody [DyLight 488]
Product Specific Notices for Goat anti-Human IgG Fc Secondary Antibody [DyLight 488]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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