Rabbit anti-Chicken IgG Fc Secondary Antibody [Alkaline Phosphatase]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72633
Conjugate
Catalog #
Key Product Details
Species Reactivity
Chicken
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Alkaline Phosphatase
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Chicken IgG F(c) fragment
Specificity
No reaction was observed against Chicken IgG F(ab).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(c) and Chicken Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(c) and Chicken Serum
Scientific Data Images for Rabbit anti-Chicken IgG Fc Secondary Antibody [Alkaline Phosphatase]
Western Blot: Rabbit anti-Chicken IgG Fc Secondary Antibody [Alkaline Phosphatase] [NBP1-72633] - Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Lane 3: Chicken IgG Fab Fragment. Lane 4: Chicken IgM Whole Molecule. Lane 5: Chicken Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(c) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgG and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.
Applications for Rabbit anti-Chicken IgG Fc Secondary Antibody [Alkaline Phosphatase]
Application
Recommended Usage
ELISA
1:5000
Immunohistochemistry
1:200 - 1:1000
Western Blot
1:500 - 1:2500
Application Notes
This product has been tested by ELISA. This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:9,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Rabbit anti-Chicken IgG Fc Secondary Antibody [Alkaline Phosphatase]
Product Specific Notices for Rabbit anti-Chicken IgG Fc Secondary Antibody [Alkaline Phosphatase]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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