Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed)

Mouse

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Polyclonal Goat IgG

Pre-adsorbed

0.5 mg/ml

Mouse IgG

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.

Polyclonal

Goat

IgG

DyLight 649 is excited at 646 (in PBS) and emits at 674 (in PBS).

Immunogen affinity purified

PBS containing 0.2% BSA

Pre-adsorbed

0.09% Sodium Azide

0.5 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C. Do not freeze.