Goat anti-Rat IgG Fc Secondary Antibody [DyLight 649] (Pre-adsorbed)

Rat

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

DyLight 649 (Excitation = 654 nm, Emission = 673 nm)

Polyclonal Goat IgG

Pre-adsorbed

0.5 mg/ml

Rat IgG-Fc Fragment

Minimum reactivity to human and mouse.

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.

Polyclonal

Goat

IgG

Immunogen affinity purified

PBS containing 0.2% BSA

Pre-adsorbed

0.09% Sodium Azide

0.5 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C. Do not freeze.