Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Human IgG Fc antiserum was solid phase adsorbed to ensure class specificity.

Reactivity Notes

Minimum reactivity to bovine, chicken, horse, mouse, rabbit, rat and sheep.

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using bovine, chicken, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. Less than 1% cross reactivity to bovine, chicken, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications for Goat anti-Human IgG Fc Secondary Antibody (Pre-adsorbed)

Application
Recommended Usage

ELISA

1:1000 - 1:20000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Western Blot

1:1000 - 1:20000
Application Notes
Suggested starting dilutions are: ELISA: Coating: 1:100 - 1:500, Primary: 1:1000 - 1:30,000. Western Blots: Colorimetric: 1:1,000 - 1:10,000, Chemiluminescent: 1:1,000 - 1:30,000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Phosphate Buffered Saline (PBS)

Format

Pre-adsorbed

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Product Documents for Goat anti-Human IgG Fc Secondary Antibody (Pre-adsorbed)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Human IgG Fc Secondary Antibody (Pre-adsorbed)

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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