Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody [FITC] (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-6253
Key Product Details
Species Reactivity
Rat
Applications
Dot Blot, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Polyclonal Goat IgG
Format
Pre-adsorbed
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Rat IgG F(c) fragment
Specificity
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse and Human Serum Proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum
Scientific Data Images for Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody [FITC] (Pre-adsorbed)
Dot Blot: Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody [FITC] (Pre-adsorbed) [NB120-6253] - Dot Blot results of Goat F(ab')2 anti-Rat IgG Fc Secondary antibody [FITC] (Pre-adsorbed).Dots are Rat F(c) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 60 min at RT.Primary Antibody: none.Secondary Antibody: Goat F(ab')2 Anti-Rat IgG Antibody FITC at 1ug/mL for 1hr at RT.Imaged with BioRad ChemiDoc, FITC filter.
Applications for Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody [FITC] (Pre-adsorbed)
Application
Recommended Usage
Flow Cytometry
1:500 - 1:2500
Fluorophore-linked immunosorbent assay
1:10000 - 1:50000
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
This product has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Format
Pre-adsorbed
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Specific Notices for Goat F(ab')2 anti-Rat IgG Fc Secondary Antibody [FITC] (Pre-adsorbed)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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