Rabbit anti-Goat IgG F(ab')2 Secondary Antibody [HRP] (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73575
Key Product Details
Species Reactivity
Goat
Applications
ELISA, Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Format
Pre-adsorbed
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Goat IgG F(ab')2 fragment
Specificity
This antibody was pre-adsorbed against Human Serum Proteins. No reaction was observed against Goat IgG F(c) or Human Serum Proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG, Goat IgG F(ab')2 and Goat Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG, Goat IgG F(ab')2 and Goat Serum
Scientific Data Images for Rabbit anti-Goat IgG F(ab')2 Secondary Antibody [HRP] (Pre-adsorbed)
ELISA: Rabbit anti-Goat IgG F(ab')2 Secondary Antibody [HRP] (Pre-adsorbed) [NBP1-73575] - ELISA results of purified Rabbit anti-Goat IgG F(ab')2 Secondary antibody [HRP] (Pre-adsorbed) tested against purified Goat IgG F(ab')2. Each well was coated in duplicate with 1.0 ug of Goat IgG F(ab')2. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.
Applications for Rabbit anti-Goat IgG F(ab')2 Secondary Antibody [HRP] (Pre-adsorbed)
Application
Recommended Usage
ELISA
1:10000 - 1:50000
Immunohistochemistry
1:500 - 1:2500
Western Blot
1:1000 - 1:10000
Application Notes
This product has been tested by ELISA. This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:62,000 of the reconstitution concentration is suggested for this product.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Format
Pre-adsorbed
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG F(ab')2
Additional IgG F(ab')2 Products
Product Specific Notices for Rabbit anti-Goat IgG F(ab')2 Secondary Antibody [HRP] (Pre-adsorbed)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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