Goat anti-Equine IgG F(ab')2 Secondary Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73524
Conjugate
Catalog #
Key Product Details
Species Reactivity
Equine
Applications
Dot Blot, ELISA, Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Horse IgG F(ab')2 fragment
Specificity
No reaction was observed against Horse IgG F(c).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Horse IgG, Horse IgG F(ab')2 and Horse Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Horse IgG, Horse IgG F(ab')2 and Horse Serum
Scientific Data Images for Goat anti-Equine IgG F(ab')2 Secondary Antibody [HRP]
ELISA: Goat anti-Equine IgG F(ab')2 Secondary Antibody [HRP] [NBP1-73524] - ELISA results of purified Goat anti-Equine IgG F(ab')2 Secondary antibody [HRP] tested against Horse IgG F(ab')2 (red) and Horse IgG (green). Each well was coated in duplicate with 1.0ug of antigen. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.
Applications for Goat anti-Equine IgG F(ab')2 Secondary Antibody [HRP]
Application
Recommended Usage
ELISA
1:50000 - 1:150000
Immunohistochemistry
1:500 - 1:2500
Western Blot
1:1000 - 1:5000
Application Notes
This product has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG F(ab')2
Additional IgG F(ab')2 Products
Product Documents for Goat anti-Equine IgG F(ab')2 Secondary Antibody [HRP]
Product Specific Notices for Goat anti-Equine IgG F(ab')2 Secondary Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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