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Rabbit anti-Bovine IgG F(ab')2 Secondary Antibody [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73497

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Bovine

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

HRP

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Bovine IgG F(ab')2 fragment

Specificity

No reaction was observed against Bovine IgG F(c).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(ab')2 and Bovine Serum

Scientific Data Images for Rabbit anti-Bovine IgG F(ab')2 Secondary Antibody [HRP]

ELISA: Rabbit anti-Bovine IgG F(ab')2 Secondary Antibody [HRP] [NBP1-73497] - ELISA Results of Purified Rabbit anti-Bovine IgG F(ab')2 Secondary antibody [HRP] tested against purified Bovine IgG F(ab')2 HRP. Each well was coated in duplicate with 1.0 ug of Bovine IgG F(ab')2. The working dilution is 1:69,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate stabilizer and TMB substrate.

Applications for Rabbit anti-Bovine IgG F(ab')2 Secondary Antibody [HRP]

Application
Recommended Usage

ELISA

1:40000 - 1:80000

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:2500

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

1:1000 - 1:10000
Application Notes
This product has been tested by ELISA. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Polyethylene Glycol (PEG-8000)

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Bovine IgG F(ab')2 Secondary Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit anti-Bovine IgG F(ab')2 Secondary Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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