Key Product Details

Species Reactivity

Mouse

Applications

Dot Blot, ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG F(ab')2 Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG F(ab')2 fragment

Specificity

No reaction was observed against Mouse IgG F(c).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody

Western Blot: Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [NBP1-72760]

Western Blot: Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [NBP1-72760]

Western Blot: Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [NBP1-72760] - WB shows detection of mouse anti-beta Actin monoclonal antibody (Catalog# NBP1-47423) using a HRP conjugated rabbit anti-mouse IgG, F(ab')2 secondary antibody (Catalog# NBP1-73640). Image from verified customer review. Image using the HRP form of this antibody.
ELISA: Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [NBP1-72760]

ELISA: Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [NBP1-72760]

ELISA: Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody [NBP1-72760] - ELISA Results of Rabbit anti-Mouse IgG F(ab')2 Secondary antibody tested against purified Mouse IgG F(ab')2. Each well was coated in duplicate with 10 ug of Mouse IgG F(ab')2. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, goat Anti-Rabbit IgG HRP conjugated and TMB substrate.

Applications for Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody

Application
Recommended Usage

ELISA

1:75000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:2000 - 1:10000
Application Notes
This product has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.