Goat F(ab')2 anti-Rabbit IgG F(ab')2 Secondary Antibody (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-6107
![Western Blot: Goat F(ab')2 anti-Rabbit IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NB120-6107]](https://resources.bio-techne.com/images/products/Goat-F-ab-2-anti-Rabbit-IgG-Secondary-Antibody-Western-Blot-NB120-6107-img0003.jpg "Western Blot: Goat F(ab')2 anti-Rabbit IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NB120-6107]")
Key Product Details
Species Reactivity
Rabbit
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
Pre-adsorbed
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Rabbit IgG F(ab')2 fragment
Specificity
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab')2 and Rabbit Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images
Western Blot: Goat F(ab')2 anti-Rabbit IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NB120-6107]
Western Blot: Goat F(ab')2 anti-Rabbit IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NB120-6107] - Lane 1: Rabbit IgG F(ab')2 Load: 100 ng per lane Primary antibody: F(ab')2 Rabbit IgG F(ab')2 Antibody Pre-Adsorbed at 1:1000 o/n at 4C Secondary antibody: DyLight 800 Donkey anti-goat at 1:20,000 for 30 min at RT Block: incubated with blocking buffer for 30 min at RT Predicted/Observed size: 28 kDa, 28 kDa Other band(s): antigen breakdown
Goat F(ab')2 anti-Rabbit IgG F(ab')2 Secondary Antibody (Pre-adsorbed)
Western Blot of Goat anti-F(ab')2 Rabbit IgG F(ab')2 Antibody Pre-Adsorbed. Lane 1: Rabbit IgG F(ab')2Applications
Application
Recommended Usage
ELISA
1:10000 - 1:50000
Immunohistochemistry
1:500 - 1:3000
Western Blot
1:1000 - 1:5000
Application Notes
This product has been tested by ELISA and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 ug of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Pre-adsorbed
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG F(ab')2
Additional IgG F(ab')2 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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