Sheep anti-Human IgD Fc Secondary Antibody

Human

Immunoelectrophoresis, Immunohistochemistry-Paraffin

Unconjugated

Polyclonal Sheep

LYOPH mg/ml

Pools of purified homogenous IgD isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this conjugate has not been tested in detail.

The reactivity of the antiserum is restricted to the Fc part of the IgD molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of the immunogen, a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with human serum containing monoclonal IgD. No precipitation reaction is obtained with purified IgG, IgA, IgM, and IgG/Fab fragments.

Polyclonal

Sheep

Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.

Whole Antiserum

Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Delipidated, heat inactivated, lyophilized, stable whole antiserum

No Preservative

LYOPH mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C. Do not freeze.