Skip to main content

Rabbit anti-Duck IgA/IgG/IgM (H+L) Secondary Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69291

Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-69291

Key Product Details

Species Reactivity

Duck

Applications

Immunoelectrophoresis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Purified normal Ig fractions isolated from pooled duck serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Reactivity Notes

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Specificity

The reactivity of the antiserum is directed to the Fc and Fab subunits of the major immunoglobulins. It does not react with any non-Ig protein in duck serum, as tested by immunoelectrophoresis and double radial immunodiffusion.

Clonality

Polyclonal

Host

Rabbit

Description

The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Applications for Rabbit anti-Duck IgA/IgG/IgM (H+L) Secondary Antibody

Application
Recommended Usage

Immunoelectrophoresis

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion use a rosette arrangement with 10 ul antiserum in a 3 mm diameter center well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Precipitation assays.

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Reconstitution

Reconstitute by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2).

Formulation

Stable whole antiserum

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgA/IgG/IgM (H+L)

Additional IgA/IgG/IgM (H+L) Products

Product Documents for Rabbit anti-Duck IgA/IgG/IgM (H+L) Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit anti-Duck IgA/IgG/IgM (H+L) Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...