Goat F(ab')2 anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody [Rhodamine] (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-6004
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence
Label
Rhodamine (Excitation = 570 nm, Emission = 590 nm)
Antibody Source
Polyclonal Goat IgG
Format
Pre-adsorbed
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse IgG IgA and IgM whole molecule
Specificity
No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum
Applications
Application
Recommended Usage
Flow Cytometry
1:500 - 1:2500
Fluorophore-linked immunosorbent assay
1:10000 - 1:50000
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Format
Pre-adsorbed
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgA/IgG/IgM (H+L)
Additional IgA/IgG/IgM (H+L) Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...