α-Amanitin
Tocris Bioscience, part of Bio-Techne | Catalog # 4025
Inhibitor of RNA polymerase II
Key Product Details
Description
Inhibitor of RNA polymerase II
Product Description
α-Amanitin is an inhibitor of RNA polymerase II. Inhibits transcription in eukaryotic cells. Binds and blocks the largest subunits of RNA polymerase II, preventing new ribonucleotides from incorporating into the nascent RNA chain. Potent amatoxin.Product Specifications
Molecular Weight
918.97
Formula
C39H54N10O14S
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
cyclo[L-Asparaginyl-4-hydroxy-L-prolyl-(R)-4,5-dihydroxy-L-isoleucyl-6-hydroxy-2-mercapto-L-tryptophylglycyl-L-isoleucylglycyl-L-cysteinyl]cyclic (4→8)-sulfide (R)-S-oxide
CAS Number
23109-05-9
PubChem ID
441541
InChI Key
CIORWBWIBBPXCG-JAXJKTSHSA-N
SMILES
O=[C@@](N[C@@](C(N[C@@H](CC2=C(S4=O)NC3=C2C=CC(O)=C3)C(NCC(N[C@]([H])5[C@@H](C)CC)=O)=O)=O)([H])[C@@H](C)[C@@H](O)CO)[C@@H]1C[C@@H](O)CN1C([C@H](CC(N)=O)NC([C@H](C4)NC(CNC5=O)=O)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 4.59 | 5 |
Preparing Stock Solutions
for α-Amanitin
The following data is based on the product molecular weight 918.97
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.05 mM | 21.76 mL | 108.82 mL | 217.63 mL |
| 0.25 mM | 4.35 mL | 21.76 mL | 43.53 mL |
| 0.5 mM | 2.18 mL | 10.88 mL | 21.76 mL |
| 2.5 mM | 0.44 mL | 2.18 mL | 4.35 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Raha Close association of RNA polymerase II and many transcription factors with Pol III genes. Proc.Natl.Acad.Sci.
- Casse The transcriptional inhibitors, actinomycin D and α-amanitin, activate the HIV-1 promoter and favor phosphorylation of the RNA polymerase II C-terminal domain. J.Biol.Chem. 1999 PMID: 10347161
Product Specific Notices for α-Amanitin
For research use only
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