Recombinant Mouse VEGF120 Protein

Name: Recombinant Mouse VEGF120 Protein
Brand: Novus Biologicals
SKU: NBP2-35188

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35188

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q00731

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all VEGF120 Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains with Met at N-terminus corresponding to VEGF120 Source: E. coli

Uniprot ID: Q00731

Amino Acid Sequence: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of VEGF120 as determined by LAL method.

Predicted Molecular Mass

28.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

VEGF120 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse VEGF120 Protein

SDS-PAGE: Recombinant Mouse VEGF120 Protein [NBP2-35188]

SDS-Page: Recombinant Mouse VEGF120 Protein [NBP2-35188]

Formulation, Preparation and Storage

NBP2-35188

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: VEGF120

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells that stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. VEGF signals through the three receptors: fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Mouse express alternately spliced isoforms of 120, 164, 182 amino acids (a.a.) in length. The VEGF120 shares 98 % a.a. sequence identity with corresponding regions of rat, 89 % with canine, feline, equine and porcine, and 87 % with human, ovine and bovine VEGF, respectively.

Gene Symbol

VEGFA

UniProt