Recombinant Human UCH-L5/UCH37 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72315

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NBP1-72315-0.1mg
NBP1-72315-0.5mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all UCH-L5/UCH37 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-329 of Human UCH-L5/UCH37

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

39.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity > 500 pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human UCH-L5/UCH37 His Protein

SDS-PAGE: Recombinant Human UCH-L5/UCH37 His Protein [NBP1-72315]

SDS-PAGE: Recombinant Human UCH-L5/UCH37 His Protein [NBP1-72315]

SDS-Page: Recombinant Human UCH-L5/UCH37 Protein [NBP1-72315] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72315
Formulation 20 mM Tris-HCl buffer (pH 8.0), 5mM DTT, 30% glycerol, 200 mM NaCl, 0.1 mM PMSF, 2 mM EDTA
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCH-L5/UCH37

Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Recombinant human UCHL5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Ubiquitin C-terminal Hydrolase L5

Alternate Names

CGI-70, INO80R, UCH37, UCHL5

Gene Symbol

UCHL5

Additional UCH-L5/UCH37 Products

Product Documents for Recombinant Human UCH-L5/UCH37 His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human UCH-L5/UCH37 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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