A customer is looking for an antigen Cardiac Troponin T for her ELISA, but it must not have a His-tag and no mercaptoethanol in the buffer. Would this be true for the antigen NBC1-28765? In addition, the customer wants to be sure that it really is a Cardiac Troponin T , because on your website some different information are given, like TNNT1 which is slow skeletal muscle.

Name: Recombinant Human Troponin T Type 2 (cardiac) His Protein
Brand: Novus Biologicals
SKU: NBC1-28765
Rating: 5 (1 reviews)

I have carried out a search and I cannot find a suitable protein that matches your customers request. Product NBC1-28765 is not a Cardiac Troponin T Protein. It is slow skeletal muscle. I am very sorry that we do not have what your customer is looking for!

Recombinant Human Troponin T Type 2 (cardiac) His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-28765

(1)

Citations (2)

Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Troponin T Type 2 (cardiac) Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-285 of Human Troponin T Type 2 (cardiac)

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

36.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 5 using NBC1-28765 in the following applications:

ELISA (1 Review)

Scientific Data Images for Recombinant Human Troponin T Type 2 (cardiac) His Protein

SDS-PAGE: Recombinant Human Troponin T Type 2 (cardiac) His Protein [NBC1-28765]

SDS-Page: Recombinant Human Troponin T Type 2 (cardiac) Protein [NBC1-28765]

Formulation, Preparation and Storage

NBC1-28765

Formulation	20 mM Tris-HCl buffer (pH 8.0), 50% glycerol, 0.5 M NaCl, 0.1 mM PMSF, 1 mM DTT, 100 mM imidazole
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin T Type 2 (cardiac)

Troponin T Type 2 (cardiac), also known as TNNT2, is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with restrictive and dilated cardiomyopathy. Recombinant human TNNT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

cardiac, CMD1D, CMPD2, dilated 1D (autosomal dominant), hypertrophic 2, MGC3889, RCM3, TnTc, troponin T type 2 (cardiac), troponin T, cardiac muscle

Gene Symbol

TNNT2

Additional Troponin T Type 2 (cardiac) Products

Product Documents for Recombinant Human Troponin T Type 2 (cardiac) His Protein

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Recombinant Human Troponin T Type 2 (cardiac) His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations
Reviews