Recombinant Human Triosephosphate isomerase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49451

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NBP1-49451-0.5mg
NBP1-49451-0.1mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Triosephosphate isomerase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-249 of Human Triosephosphate isomerase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Triosephosphate isomerase His Protein

SDS-PAGE: Recombinant Human Triosephosphate isomerase His Protein [NBP1-49451]

SDS-PAGE: Recombinant Human Triosephosphate isomerase His Protein [NBP1-49451]

SDS-Page: Triosephosphate isomerase Protein [NBP1-49451] - 15% SDS-PAGE (3 ug).

Formulation, Preparation and Storage

NBP1-49451
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Triosephosphate isomerase

TPI1 (Triosephosphate isomerase) belongs to the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Recombinant human TPI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EC 5.3.1.1, MGC88108, TIM, TPI, triosephosphate isomerase, Triose-phosphate isomerase, triosephosphate isomerase 1

Gene Symbol

TPI1

Additional Triosephosphate isomerase Products

Product Documents for Recombinant Human Triosephosphate isomerase His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Triosephosphate isomerase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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