Amino Acid Sequence: MDGTRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGKTAF HQEQGHQLLK KHLDTFLKGK SGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIQEFF TEQNLSYSEE PITEIPGTKV FKSSSGNISL YCCSIFDLPR TNIGKFDMIW DRGALVAINP GDRKCYADTM FSLLGKKFQY LLCVLSYDPT KHPGPPFYVP HAEIERLFGK ICNIRRLEKV DAFEERHKSW GIDCLFEKLY LLTEK

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TPMT Protein

SDS-PAGE: Recombinant Human TPMT Protein [NBC1-18525]

SDS-Page: Recombinant Human TPMT Protein [NBC1-18525] - TPMT, 28 kDa (245 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-18525

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.2 mM PMSF, 2 mM EDTA
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TPMT

TPMT, thiopurine S-methyltransferase, is a cytosolic enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine and azathioprine are used as chemotherapeutic agents. TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity. Recombinant human TPMT was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Thiopurine S-methyltransferase

Alternate Names

EC 2.1.1.67

Gene Symbol

TPMT

Additional TPMT Products

Product Documents for Recombinant Human TPMT Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human TPMT Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations
Reviews

Recombinant Human TPMT Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18525

Key Product Details

Source

Conjugate

Applications

View all TPMT Proteins and Enzymes »

Product Specifications

Description