Recombinant Human TFIIE beta His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98980

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NBP1-98980-50ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all TFIIE beta Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-291 of Human TFIIE beta

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMDPSLL RERELFKKRA LSTPVVEKRS ASSESSSSSS KKKKTKVEHG GSSGSKQNSD HSNGSFNLKA LSGSSGYKFG VLAKIVNYMK TRHQRGDTHP LTLDEILDET QHLDIGLKQK QWLMTEALVN NPKIEVIDGK YAFKPKYNVR DKKALLRLLD QHDQRGLGGI LLEDIEEALP NSQKAVKALG DQILFVNRPD KKKILFFNDK SCQFSVDEEF QKLWRSVTVD SMDEEKIEEY LKRQGISSMQ ESGPKKVAPI QRRKKPASQK KRRFKTHNEH LAGVLKDYSD ITSSK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

35.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TFIIE beta His Protein

SDS-PAGE: Recombinant Human TFIIE beta His Protein [NBP1-98980]

SDS-PAGE: Recombinant Human TFIIE beta His Protein [NBP1-98980]

SDS-Page: Recombinant Human TFIIE beta Protein [NBP1-98980] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-98980
Formulation 20 mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1 M NaCl, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFIIE beta

GTF2E2, also known as TFIIE, is essential for transcription initiation by RNA polymerase II in conjunction with other general factors. TFIIE is a heterotetramer containing two subunits of relative molecular mass 57,000 (TFIIE-alpha) and two of 34,000 (TFIIE-beta). TFIIE-beta is required in conjunction with TFIIE-alpha for transcription initiation. GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Recombinant human GTF2E2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

FE, General transcription factor IIE subunit 2, general transcription factor IIE, polypeptide 2 (beta subunit, 34kD), general transcription factor IIE, polypeptide 2, beta 34kDa, TF2E2TFIIE-beta, TFIIE-B, transcription initiation factor IIE subunit beta

Gene Symbol

GTF2E2

Additional TFIIE beta Products

Product Documents for Recombinant Human TFIIE beta His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human TFIIE beta His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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