Recombinant Human TFB1M His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72409

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NBP1-72409-250ug
NBP1-72409-50ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all TFB1M Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-346 of Human TFB1M

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQAAKQLSQN FLLDLRLTDK IVRKAGNLTN AYVYEVGPGP GGITRSILNA DVAELLVVEK DTRFIPGLQM LSDAAPGKLR IVHGDVLTFK VEKAFSESLK RPWEDDPPNV HIIGNLPFSV STPLIIKWLE NISCRDGPFV YGRTQMTLTF QKEVAERLAA NTGSKQRSRL SVMAQYLCNV RHIFTIPGQA FVPKPEVDVG VVHFTPLIQP KIEQPFKLVE KVVQNVFQFR RKYCHRGLRM LFPEAQRLES TGRLLELADI DPTLRPRQLS ISHFKSLCDV YRKMCDEDPQ LFAYNFREEL KRRKSKNEEK EEDDAENYRL

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

38.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TFB1M His Protein

SDS-PAGE: Recombinant Human TFB1M His Protein [NBP1-72409]

SDS-PAGE: Recombinant Human TFB1M His Protein [NBP1-72409]

SDS-Page: Recombinant Human TFB1M Protein [NBP1-72409] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72409
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFB1M

TFB1M belongs to the methyltransferase superfamily. TFB1M is a dimethyltransferase that methylates mitochondrial 12S rRNA at the conserved stem loop. This protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial gene expression. TFB1M stimulates transcription independently of the methyltransferase activity. Recombinant human TFB1M protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

CGI75, CGI-75, dimethyladenosine transferase 1, mitochondrial, EC 2.1.1, EC 2.1.1.-, h-mtTFB, h-mtTFB1, homolog of yeast mitochondrial transcription factor B, hTFB1M, Mitochondrial 12S rRNA dimethylase 1, mitochondrial dimethyladenosine transferase 1, Mitochondrial transcription factor B1, mtTFB, mtTFB1, S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1, transcription factor B1, mitochondrial

Gene Symbol

TFB1M

Additional TFB1M Products

Product Documents for Recombinant Human TFB1M His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human TFB1M His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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