I want to know which TAZ primary antibody is best produced for WB (human and mouse)!

Name: Recombinant Human TAZ/WWTR1 His Protein
Brand: Novus Biologicals
SKU: NBP2-22949

I would recommend this TAZ antibody: NB110-58359. This is also comes in a sample size.

Recombinant Human TAZ/WWTR1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22949

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all TAZ/WWTR1 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-400 of Human TAZ/WWTR1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNPASAP PPLPPPGQQV IHVTQDLDTD LEALFNSVMN PKPSSWRKKI LPESFFKEPD SGSHSRQSST DSSGGHPGPR LAGGAQHVRS HSSPASLQLG TGAGAAGSPA QQHAHLRQQS YDVTDELPLP PGWEMTFTAT GQRYFLNHIE KITTWQDPRK AMNQPLNHMN LHPAVSSTPV PQRSMAVSQP NLVMNHQHQQ QMAPSTLSQQ NHPTQNPPAG LMSMPNALTT QQQQQQKLRL QRIQMERERI RMRQEELMRQ EAALCRQLPM EAETLAPVQA AVNPPTMTPD MRSITNNSSD PFLNGGPYHS REQSTDSGLG LGCYSVPTTP EDFLSNVDEM DTGENAGQTP MNINPQQTRF PDFLDCLPGT NVDLGTLESE DLIPLFNDVE SALNKSEPFL TWL

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TAZ/WWTR1 His Protein

SDS-PAGE: Recombinant Human TAZ/WWTR1 His Protein [NBP2-22949]

SDS-Page: Recombinant Human TAZ/WWTR1 Protein [NBP2-22949]

Formulation, Preparation and Storage

NBP2-22949

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TAZ/WWTR1

WWTR1 is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Recombinant human WWTR1 protein, fused to His-tag at N-terminus, was expressed in E.coli .

Long Name

Transcriptional Coactivator with PDZ-binding Motif

Alternate Names

WWTR1

Gene Symbol