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Recombinant Streptavidin Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35315

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NBP2-35315-10mg

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A biologically active protein to Streptavidin.

Source: Recombinant streptavidin from Streptomyces avidinii, produced in Escherichia coli.Amino Acid Sequence: MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Streptavidin as determined by LAL method.

Predicted Molecular Mass

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

> 17 U/mg (one unit binds 1 ug D-biotin at pH 8.9)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Streptavidin Protein

SDS-PAGE: Recombinant Streptavidin Protein [NBP2-35315]

SDS-PAGE: Recombinant Streptavidin Protein [NBP2-35315]

SDS-Page: Recombinant Bacterial Streptavidin Protein [NBP2-35315] - Streptavidin Protein [NBP2-35315]

Formulation, Preparation and Storage

NBP2-35315
Formulation Lyophilized in 10 mM potassium phosphate buffer pH 6.5.
Preservative No Preservative
Concentration Lyoph
Reconstitution Reconstitute with distilled water or saline.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: Streptavidin

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.

Alternate Names

SA V1; SA V2; Streptavidin V1; Streptavidin V2

Additional Streptavidin Products

Product Documents for Recombinant Streptavidin Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Streptavidin Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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