Recombinant Human ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52038

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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all ST6 Gal Sialyltransferase 1/ST6GAL1 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 27-406 of Human ST6 Gal Sialyltransferase 1/ST6GAL1/CD75

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSKEKKKGS YYDSFKLQTK EFQVLKSLGK LAMGSDSQSV SSSSTQDPHR GRQTLGSLRG LAKAKPEASF QVWNKDSSSK NLIPRLQKIW KNYLSMNKYK VSYKGPGPGI KFSAEALRCH LRDHVNVSMV EVTDFPFNTS EWEGYLPKES IRTKAGPWGR CAVVSSAGSL KSSQLGREID DHDAVLRFNG APTANFQQDV GTKTTIRLMN SQLVTTEKRF LKDSLYNEGI LIVWDPSVYH SDIPKWYQNP DYNFFNNYKT YRKLHPNQPF YILKPQMPWE LWDILQEISP EEIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC YYYQKFFDSA CTMGAYHPLL YEKNLVKHLN QGTDEDIYLL GKATLPGFRT IHC

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 5 using NBP2-52038 in the following applications:

Scientific Data Images

SDS-PAGE: Recombinant Human ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 His Protein [NBP2-52038]

SDS-PAGE: Recombinant Human ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 His Protein [NBP2-52038]

SDS-Page: Recombinant Human ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Protein [NBP2-52038]

Formulation, Preparation and Storage

NBP2-52038
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ST6 Gal Sialyltransferase 1/ST6GAL1

CD75 is encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi apparatus but which can be proteolytically processed to a soluble form, is involved in the generation of the cell surface carbohydrate determinants and differentiation antigens HB6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. This antibody recognizes the CD75 cell surface antigen, an alpha 2,6 sialyated carbohydrate epitope expressed by mature B cells, especially germinal centre B cells, red blood cells and some epithelial cells.

Long Name

ST6 beta-Galactoside alpha-2,6-Sialyltransferase 1

Alternate Names

Alpha 2,6-ST, B-cell antigen CD75, CD75, SIAT1, ST6N

Gene Symbol

ST6GAL1

Additional ST6 Gal Sialyltransferase 1/ST6GAL1 Products

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Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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