Recombinant Human sRank Receptor/TNFRSF11A Protein

Name: Recombinant Human sRank Receptor/TNFRSF11A Protein
Brand: Novus Biologicals
SKU: NBP2-35272

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35272

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Product Datasheet / COA

Catalog #

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9Y6Q6

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all sRank Receptor/TNFRSF11A Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 174 amino acids corresponding to sRank Receptor/TNFRSF11A Source: E. coli

Uniprot ID: Q9Y6Q6

Amino Acid Sequence: QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of sRank Receptor/TNFRSF11A as determined by LAL method.

Predicted Molecular Mass

19.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

sRank Receptor/TNFRSF11A protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x 10^4 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human sRank Receptor/TNFRSF11A Protein

SDS-PAGE: Recombinant Human sRank Receptor/TNFRSF11A Protein [NBP2-35272]

SDS-Page: Human sRank Receptor/TNFRSF11A Protein [NBP2-35272]

Formulation, Preparation and Storage

NBP2-35272

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: sRank Receptor/TNFRSF11A

RANK Ligand and RANK Receptor are members of the TNF superfamily that play an important role in the regulation of specific immunity and bone turnover. RANK Receptor was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naive T cell proliferation and to promote the survival of RANK + T cells. RANK is also expressed in a variety of tissues including skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. The RANK/RANKL interaction is important in the regulation of osteoclastogenesis and in dendritic-cellmediated T cell immune responses. Impairments in RANK signaling have been implicated in the induction of expansile osteolysis and Paget disease of bone (PDB2). Recombinant human sRANK receptor is a 19.1 kDa polypeptide containing the TNFR homologous cysteine rich portion of the extracellular domain of RANK receptor (174 amino acid residues).

Alternate Names

CD265, CD265 antigen, FEO, loss of heterozygosity, 18, chromosomal region 1, OFE, OPTB7, Osteoclast differentiation factor receptor, PDB2, Receptor activator of NF-KB, receptor activator of nuclear factor-kappa B, TRANCER, tumor necrosis factor receptor superfamily member 11A, tumor necrosis factor receptor superfamily, member 11a, activator of NFKB, tumor necrosis factor receptor superfamily, member 11a, NFKB activator

Gene Symbol

TNFRSF11A

UniProt