Recombinant Human Spermine synthase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78857

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NBP1-78857-50ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Spermine synthase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-366 of Human Spermine synthase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAAARH STLDFMLGAK ADGETILKGL QSIFQEQGMA ESVHTWQDHG YLATYTNKNG SFANLRIYPH GLVLLDLQSY DGDAQGKEEI DSILNKVEER MKELSQDSTG RVKRLPPIVR GGAIDRYWPT ADGRLVEYDI DEVVYDEDSP YQNIKILHSK QFGNILILSG DVNLAESDLA YTRAIMGSGK EDYTGKDVLI LGGGDGGILC EIVKLKPKMV TMVEIDQMVI DGCKKYMRKT CGDVLDNLKG DCYQVLIEDC IPVLKRYAKE GREFDYVIND LTAVPISTSP EEDSTWEFLR LILDLSMKVL KQDGKYFTQG NCVNLTEALS LYEEQLGRLY CPVEFSKEIV CVPSYLELWV FYTVWKKAKP

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

43.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Spermine synthase His Protein

SDS-PAGE: Recombinant Human Spermine synthase His Protein [NBP1-78857]

SDS-PAGE: Recombinant Human Spermine synthase His Protein [NBP1-78857]

SDS-Page: Recombinant Human Spermine synthase His Protein [NBP1-78857] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-78857
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 100 mM NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spermine synthase

SMS (Spermine synthase) belongs to the spermidine/spermine synthase family. It is an enzyme that converts spermidine into spermine. This enzyme is required for normal viability, growth and fertility involved in polyamine metabolism. Defects in SMS are the cause of Snyder-Robinson syndrome (SRS), also known as X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive. Recombinant human SMS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EC 2.5.1.22, MRSRSpS, Snyder-Robinson X-linked mental retardation syndrome, Spermidine aminopropyltransferase, spermine synthase, SPMSYSRS

Gene Symbol

SMS

Additional Spermine synthase Products

Product Documents for Recombinant Human Spermine synthase His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Spermine synthase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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