Recombinant Human SOD2/Mn-SOD His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41138

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NBP1-41138
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all SOD2/Mn-SOD Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-222 of Human SOD2/Mn-SOD

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 1,000 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SOD2/Mn-SOD His Protein

SDS-PAGE: Recombinant Human SOD2/Mn-SOD His Protein [NBP1-41138]

SDS-PAGE: Recombinant Human SOD2/Mn-SOD His Protein [NBP1-41138]

SDS-Page: Recombinant Human SOD2/Mn-SOD Protein [NBP1-41138] - Superoxide Dismutase 2 Protein SOD2, 24.4kDa (219aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-41138
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOD2/Mn-SOD

Superoxide Dismutase 2 is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial matrix protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this protein have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Recombinant human Superoxide Dismutase 2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Superoxide Dismutase-2

Alternate Names

IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial

Gene Symbol

SOD2

Additional SOD2/Mn-SOD Products

Product Documents for Recombinant Human SOD2/Mn-SOD His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human SOD2/Mn-SOD His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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