Recombinant Human SIAH1 His Protein

Name: Recombinant Human SIAH1 His Protein
Brand: Novus Biologicals
SKU: NBP2-23470

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23470

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all SIAH1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 90-282 of Human SIAH1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSVANSVLF PCKYASSGCE ITLPHTEKAD HEELCEFRPY SCPCPGASCK WQGSLDAVMP HLMHQHKSIT TLQGEDIVFL ATDINLPGAV DWVMMQSCFG FHFMLVLEKQ EKYDGHQQFF AIVQLIGTRK QAENFAYRLE LNGHRRRLTW EATPRSIHEG IATAIMNSDC LVFDTSIAQL FAENGNLGIN VTISMC

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

24.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SIAH1 His Protein

SDS-PAGE: Recombinant Human SIAH1 His Protein [NBP2-23470]

SDS-Page: SIAH1 Protein [NBP2-23470]

Formulation, Preparation and Storage

NBP2-23470

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 40% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIAH1

E3 ubiquitin-protein ligase SIAH1, also known as SIAH1, is a member of the seven in absentia homolog (SIAH) family. SIAH1 is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. SIAH1 contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. Recombinant human SIAH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

E3 ubiquitin-protein ligase SIAH1, EC 6.3.2, EC 6.3.2.-, FLJ08065, hSIAH1, HUMSIAH, Seven in absentia homolog 1, seven in absentia homolog 1 (Drosophila), Siah-1, SIAH1A, Siah-1a

Gene Symbol

SIAH1

Additional SIAH1 Products

Product Documents for Recombinant Human SIAH1 His Protein

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COA

Product Specific Notices for Recombinant Human SIAH1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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