Recombinant SARS-CoV-2 nsp13 His (N-Term) Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07081

Novus Biologicals, part of Bio-Techne
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NBP3-07081
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Key Product Details

Tag

His (N-Term)

Conjugate

Unconjugated

Applications

SDS-PAGE

View all SARS-CoV-2 nsp13 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a His (N-Term) tag corresponding to the amino acids Ala5325-Gln5925 of SARS-CoV-2 nsp13

Source: E.coli

Accession: QHD43415.1

Purity

>90%, by SDS-PAGE quantitative densitometry by Coomassie Blue Staining

Predicted Molecular Mass

69.15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant SARS-CoV-2 nsp13 His (N-Term) Protein

SDS-PAGE: Recombinant SARS-CoV-2 nsp13 His (N-Term) Protein [NBP3-07081]

SDS-PAGE: Recombinant SARS-CoV-2 nsp13 His (N-Term) Protein [NBP3-07081]

SDS-Page: Recombinant SARS-CoV-2 nsp13 His (N-Term) Protein [NBP3-07081] - SDS-PAGE Image

Formulation, Preparation and Storage

NBP3-07081
Formulation PBS, pH 7.5, 0.02% NLS
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp13

SARS-CoV-2 Nonstructural Protein 13 (NSP13) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP13 is 601 amino acids (aa) with a theoretical molecular weight of 66.9 kDa (1-3). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP13 has 99.8% sequence identity and 100% sequence similarity, with only one amino acid residue difference between the two proteins (1). NSP13 is suggested to have helicase activity and unwinds RNA (1,3). Furthermore, NSP13 is suggested to interact with NSP12 and through protein-protein interactions, NSP12 enhances NSP13's helicase activity (1,3,4). Additionally, a SARS-CoV-2 interactome study reveals NSP13's involvement in several processes including transcriptional regulation, Golgi organization, protein kinase A signaling, and centrosome complex formation (2). Given its role in centrosome organization, NSP13 is a possible molecular link for the loss of smell, called anosmia, due to the olfactory cilia defects that can occur because of COVID-19 infection (5). NSP13 also interacts with innate immune pathways including the interferon pathway and NF-kappaB pathway (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Romano, M., Ruggiero, A., Squeglia, F., Maga, G., & Berisio, R. (2020). A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping. Cells. https://doi.org/10.3390/cells9051267

5. Li, W., Li, M., & Ou, G. (2020). COVID-19, cilia, and smell. The FEBS journal. https://doi.org/10.1111/febs.15491

Alternate Names

helicase, Non-structural protein 13

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp13 Products

Product Documents for Recombinant SARS-CoV-2 nsp13 His (N-Term) Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant SARS-CoV-2 nsp13 His (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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