Recombinant Zebrafish Ephrin-B2 Fc Chimera Protein, CF

Name: Recombinant Zebrafish Ephrin-B2 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1088-B2

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1088-B2

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	O73874
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications for Recombinant Zebrafish Ephrin-B2 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived zebrafish Ephrin-B2 protein

Zebrafish Ephrin-B2 (Leu25 - Ala 222) Accession # O73874	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu25

Predicted Molecular Mass

48.5 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to compete with biotinylated rmEphrin-B2/Fc Chimera (R&D Systems, Catalog # BT496) for binding with immobilized rmEph-B2/Fc Chimera in a functional ELISA assay.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1088-B2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-B2

Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1, is a 40 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B2 preferentially interacts with receptors in the EphB family (1, 2). Mature zebrafish Ephrin-B2 consists of a 201 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 87 aa cytoplasmic domain (3). Within the ECD, zebrafish Ephrin-B2 shares 56% aa sequence identity with human, mouse, and rat Ephrin-B2. Ephrin-B2 is expressed presynaptically on neurons (4, 5). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (4 - 6). Ephrin-B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (7, 8). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (7 - 9). Ephrin-B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (10, 11). It is upregulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (12 - 14). It functions as a cellular entry receptor for Hendra and Nipah viruses (15). Ephrin-B2 is also important for the separation of the urinary and intestinal tracts during development (16).

Item	Value
References	Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165. Durbin, L. et al. (1998) Genes Dev. 12:3096. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. 106:20487. Zhao, J. et al. (2010) Mol. Pain 6:77. Lin, K.-T. et al. (2008) J. Biol. Chem. 283:28969. Foo, S.S. et al. (2006) Cell 124:161. Wang, Y. et al. (2010) Nature 465:483. Sawamiphak, S. et al. (2010) Nature 465:487. Pfaff, D. et al. (2008) J. Cell Sci. 121:3842. Yu, G. et al. (2003) J. Immunol. 171:106. Meyer, S. et al. (2005) Int. J. Oncol. 27:1197. Nakada, M. et al. (2010) Int. J. Cancer 126:1155. Liu, W. et al. (2004) Br. J. Cancer 90:1620. Bonaparte, M.I. et al. (2005) Proc. Natl. Acad. Sci. 102:10652. Dravis, C. et al. (2004) Dev. Biol. 271:272.
Entrez Gene IDs	1948 (Human); 13642 (Mouse); 30219 (Zebrafish)
Alternate Names	EFNB2, ELF-2, EPLG5, Ephrin-B2, EphrinB2, HTKL, Htk-L, LERK-5, LERK5, NLERK-1, ephrin B2

Citations for Recombinant Zebrafish Ephrin-B2 Fc Chimera Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Zebrafish Ephrin-B2 Fc Chimera Protein, CF include:

Species: Zebrafish
Sample Types: Whole Tissue
Applications: IHC

Hendricks M et al. (2007), Disruption of Esrom and Ryk identifies the roof plate boundary as an intermediate target for commissure formation. Mol. Cell. Neurosci., 37(2):271-83.
PMID: 18060805
Species: Zebrafish
Sample Types: Whole Tissue
Applications: Binding Assay

D&amp;apos;Souza J et al. (2005), Formation of the retinotectal projection requires Esrom, an ortholog of PAM (protein associated with Myc). Development, 132(2):247-56.
PMID: 15590740

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