Recombinant Zebrafish BMP-4 Protein

Name: Recombinant Zebrafish BMP-4 Protein
Brand: R&D Systems
SKU: 1128-BM

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1128-BM

Citations (3)

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Key Product Details

Source:	E. coli
Accession #:	NP_571417
Applications:	Bioactivity

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Product Specifications for Recombinant Zebrafish BMP-4 Protein

Source	E. coli-derived zebrafish BMP-4 protein Ser288-Arg400, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	12.9 kDa
Activity	Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 0.2-1 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1128-BM

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1128-BM/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-4

BMP-4 is one of at least 15 structurally and functionally-related BMPs, which are members of the transforming growth factor-beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to have roles in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta superfamily proteins, BMPs are highly conserved across animal species. Zebrafish BMP-4 protein shares 73% amino acid sequence identity with human and murine BMP-4, and 86% amino acid sequence identity with zebrafish BMP-2b. Zebrafish BMP-4 mRNA is detected throughout embryonic development and has been detected in tissues of adult fish, including the brain, heart, digestive tracts, testes, and jaw. BMP-4 has been shown to play roles in directing zebrafish heart looping and in ear development. The combined expression pattern of zBMP-2a/2b/4 coincides with areas where BMP-2/4 expression would be found in other vertebrates. Biologically active BMP-4 is a disulfide-linked homodimer of the carboxy-terminal 113 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond.

Item	Value
References	Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49. Hwang, S.P. et al. (1997) DNA Cell Biol. 16:1003. Martinez-Barbera, J.P. et al. (1997) Gene 197:53. Mowbray, C. et al. (2001) Mech Dev. 108:179. Chen J.N. et al. (1997) Development 124:4373.
Long Name	Bone Morphogenetic Protein 4
Entrez Gene IDs	652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
Alternate Names	BMP-2B, BMP-4, BMP2B, BMP2B1, BMP2BMCOPS6, BMP4, Bone morphogenetic protein 2B, DVR4, MCOPS6, OFC11, ZYME, bone morphogenetic protein 4

Citations for Recombinant Zebrafish BMP-4 Protein (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Zebrafish BMP-4 Protein include:

Species: Phallusia mammillata
Sample Types: Embryos
Applications: Bioassay

R Chowdhury et al. (2022), Highly distinct genetic programs for peripheral nervous system formation in chordates Bmc Biology, 20(1):152.
PMID: 35761237
Species: Zebrafish
Sample Types: In Vivo
Applications: In Vivo

Esterberg R et al. (2008), Tailbud-derived Bmp4 drives proliferation and inhibits maturation of zebrafish chordamesoderm. Development, 135(23):3891-901.
PMID: 18948415
Species: Zebrafish
Sample Types: In Vivo
Applications: In Vivo

Denes AS et al. (2007), Molecular architecture of annelid nerve cord supports common origin of nervous system centralization in bilateria. Cell, 129(2):277-88.
PMID: 17448990

Recombinant Zebrafish BMP-4 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Zebrafish BMP-4 Protein

Formulation, Preparation and Storage

Carrier Free

1128-BM

Carrier

1128-BM/CF

Carrier Free

Reconstitution Calculator

Background: BMP-4

Citations for Recombinant Zebrafish BMP-4 Protein (3)

Product Documents for Recombinant Zebrafish BMP-4 Protein

Product Datasheets

COA

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