Recombinant X. campestris beta(1-3)-Galactosidase, CF

R&D Systems, part of Bio-Techne | Catalog # 5704-GH

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5704-GH-050
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Key Product Details

  • R&D Systems E. coli-derived Recombinant X. campestris beta(1-3)-Galactosidase (5704-GH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession #

NP_638243

Conjugate

Unconjugated

Applications

Enzyme Activity

View all beta (1-3)-Galactosidase Proteins and Enzymes »

Product Specifications

Source

E. coli-derived x. campestris beta (1-3)-Galactosidase protein
Ser25-Glu613 with an N-terminal Met and 6-His tag
Accession # NP_638243

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

67 kDa

SDS-PAGE

63 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside.
The specific activity is >14,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5704-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: beta (1-3)-Galactosidase

The majority of secreted and membrane proteins are glycosylated (1, 2). Proper glycosylation might be critical for protein folding and biological functions (3, 4). Galactoside is an essential sugar commonly found on various glycan conjugates and galactosidases are among the earliest enzymes to be studied (5). beta1‑3 Galactosidase from Xanthomanas capestris is a useful tool for removing beta1-3 linked galactosides from the non-reducing terminus of glycoconjugates (6, 7).

References

  1. Lis, H. and Sharon, N. (1993) Eur. J. Biochem. 218:1.
  2. Hart, G.W. (1992) Curr. Opin. Cell Biol. 4:1017.
  3. Dwek, R.A. (1995) Biochem. Soc. Trans. 23:1.
  4. Wormald, M.R. and Dwek, R.A. (1999) Structure 7:R155.
  5. Hood, J.M. et al. (1977) Proc. Natl. Acad. Sci. USA 75:113.
  6. Taron, C. et al. (1995) Glycobiology 5:603.
  7. Glasgow, L. et al. (1977) J. Biol. Chem. 252:8615.

Alternate Names

beta (13)Galactosidase

UniProt

NP_638243

Additional beta (1-3)-Galactosidase Products

Product Documents for Recombinant X. campestris beta(1-3)-Galactosidase, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant X. campestris beta(1-3)-Galactosidase, CF

For research use only

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