Recombinant Viral HCMV IL-10 Protein

Name: Recombinant Viral HCMV IL-10 Protein
Brand: R&D Systems
SKU: 117-VL
Price: 417 USD

Catalog # 117-VL | R&D Systems, Inc. a Bio-Techne Brand

Citations (10)

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Key Product Details

Source:	E. coli
Accession #:	AAF63437
Structure / Form:	Noncovalently-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Viral HCMV IL-10 Protein

Source	E. coli-derived viral IL-10 protein Ala26-Lys176
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala26
Predicted Molecular Mass	17.6 kDa
Activity	Measured by its ability to inhibit LPS-induced IL‑1 beta secretion by human peripheral blood lymphocytes (PBL). The ED₅₀ for this effect is 0.1-0.6 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

117-VL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄ and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

117-VL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄ and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10

Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen‑stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).

Item	Value
References	Moore, K.W. et al. (2001) Annu. Rev. Immunol. 19:683. Kotenko, S.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1695. Spencer, J.V. et al. (2002) J. Virol. 76:1285.
Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names	CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL-10, IL-10MGC126451, IL10, IL10A, TGIF, interleukin 10, interleukin-10

Citations for Recombinant Viral HCMV IL-10 Protein (10)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Viral HCMV IL-10 Protein include:

Species: Human
Sample Types: Plasma
Applications: ELISA (Standard)

S Waters et al. (2021-11-18), Challenging the Conventional Interpretation of HCMV Seronegativity Microorganisms, 2021-19(11).
PMID: 34835508
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CA Valle Oseg et al. (2017-02-13), Human cytomegalovirus interleukin-10 enhances matrigel invasion of MDA-MB-231 breast cancer cells Cancer Cell Int, 2017-017(0):24.
PMID: 28228690
Sample Types: N/A
Applications: ELISA (Standard)

J Stuchl? et al. (2017-01-05), Common Variable Immunodeficiency patients with a phenotypic profile of immunosenescence present with thrombocytopenia Sci Rep, 2017-07(0):39710.
PMID: 28054583
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Bishop R et al. (2015-01-01), Human Cytomegalovirus interleukin-10 promotes proliferation and migration of MCF-7 breast cancer cells. Cancer Cell Microenviron, 2015-02(1).
PMID: 26023679
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Valle Oseguera et al. (2014-02-10), cmvIL-10 stimulates the invasive potential of MDA-MB-231 breast cancer cells. PLoS ONE, 2014-09(2):e88708.
PMID: 24520416
Sample Types: N/A
Applications: Western Blot

Forster S et al. (2013-10-05), Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct. BMC Biotechnol, 2013-113(0):82.
PMID: 24093616
Sample Types: N/A
Applications: ELISA (Standard)

Dziurzynski K et al. (2011-04-13), Glioma-associated cytomegalovirus mediates subversion of the monocyte lineage to a tumor propagating phenotype. Clin. Cancer Res., 2011-017(14):4642-9.
PMID: 21490182
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Chang WL et al. (2009-06-13), Human cytomegalovirus suppresses type I interferon secretion by plasmacytoid dendritic cells through its interleukin 10 homolog. Virology, 2009-0390(2):330-7.
PMID: 19524994
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gruber SG et al. (2008-12-01), Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1. Eur. J. Immunol., 2008-138(12):3365-75.
PMID: 19016528
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

van Putten SM et al. (2008-11-26), The downmodulation of the foreign body reaction by cytomegalovirus encoded interleukin-10. Biomaterials, 2008-130(5):730-5.
PMID: 19038442

Recombinant Viral HCMV IL-10 Protein

Catalog # 117-VL | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Viral HCMV IL-10 Protein

Formulation, Preparation and Storage

Carrier Free

117-VL

Carrier

117-VL/CF

Carrier Free

Reconstitution Calculator

Background: IL-10

Citations for Recombinant Viral HCMV IL-10 Protein (10)

Product Documents for Recombinant Viral HCMV IL-10 Protein

Product Datasheets

COA

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SDS

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